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In the primary yellowish-white sector of new shoots in Wogon-Sugi plants, there is the possibility that matK of the mutated version lacks or has insufficient function as a result of the frameshift mutation, and that genes containing group II introns might not be spliced.
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To confirm that Lmna−/−-siGank have insufficient pRB function to mediate cell cycle arrest, GFP-p16ink4a was introduced and BrdU incorporation was monitored.
Consistently, siLmna-p14arf cells remained refractory to p16ink4a suggesting that the cells had insufficient pRB function to regulate the cell cycle (Figure 5B).
To test if the Lmna+/+-Gank cells have insufficient pRB function to maintain proper cell cycle regulation, we introduced GFP-p16ink4a and examined the proliferative state of the cells by scoring BrdU incorporation into DNA over a 6 hr incubation period.
Prior to embolia none of the patients had insufficient heart function or irregular heart rhythms.
Lucknow has insufficient transport infrastructure.
It only has insufficient urban water transport.
Rep. of Korea has insufficient supply of energy resource.
Yemen has insufficient progress towards MDG4 [ 22].
For example, cognitive interviews can be conducted to clarify whether substance abusers have insufficient cognitive functioning by which to understand negatively worded items.
Similarly, for patients with erections firm enough for intercourse at baseline, the risk of having insufficient erectile functions at 1 year after treatment was higher after RP (89%) than after BT (57%) or EBRT (33%, P<0.001).
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