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Given the difficulty or impossibility of culturing most pathogenic species, the use of next-generation sequencing has greatly enabled their identification.
The genome sequence combined with expression data has greatly enabled characterization of many protein-coding genes [ 26, 27] as well as the molecular machinery required for RNA silencing [ 28, 29].
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In recent years, work in this area has been greatly enabled by rapid developments in Geographic Information Systems, which have facilitated the management and use of spatial data for analytic epidemiology.
The ability to examine gene regulation in living cells has been greatly enabled by the development of chromatin immunoprecipitation (ChIP) methodology.
Understanding genome to phenotype linkages has been greatly enabled by genomic sequencing.
Identification of new oncogenes, tumor suppressor genes, and pathways has been greatly enabled by whole genome or exon DNA sequencing and mRNA profiling technologies.
The availability of high-throughput genotyping technologies has greatly advanced biomedical research, enabling us to detect genetic variations that are associated with the risk of diseases with much finer resolution than before.
The molecular typing of Mtb has greatly improved knowledge of TB epidemiology and enabled molecular guided control of the disease.
The genetic typing of mycobacteria has greatly improved knowledge about tuberculosis epidemiology and enabled a molecular-guided control of the disease.
These results suggest that, compared with free ICG, rNGO-PEG/ICG has greatly enhanced the permeability and retention (EPR) effect to enable effective imaging of tumor in vivo.
The successful culture of intestinal organoids has greatly enhanced our understanding of intestinal stem cell physiology and enabled the generation of novel intestinal disease models.
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