For example, Davis has described the modification of cysteine 1 to generate dehydroalanine 2, which can then be used for bioconjugation in a range of protein substrates, including a single cysteine mutant (S156C) of the protease subtilisin.
Other akynyl sterols have been previously used in conjunction with CuAAC to identify cholesterol-interacting proteins, or cholesterol metabolism and cellular localisation; however, none of these reports has described covalent modification of proteins by alkynyl sterols.
Previous work has described histone modifications associated with nuclei transplanted to mammalian eggs [ 4, 5], with embryonic (ES) hybrid cells [ 3], or with induced pluripotent stem cells [ 6].
We have recently described a modification (Veil of Aphrodite) designed to preserve the lateral prostatic fascia (LPF) during robotic prostatectomy.
We have previously described a modification to this gap detection approach in which we measured gap-induced PPI of the Preyer reflex (Berger et al., 2013).
The US Department of Justice (DOJ), which has pushed for the rule change since 2013, has described it as a minor modification needed to modernize the criminal code for the digital age, and has said it would not permit searches or seizures that are not already legal.
The U.S. Justice Department, which has pushed for the rule change since 2013, has described it as a minor modification needed to modernize the criminal code for the digital age, and has said it would not permit searches or seizures that are not already legal.
To date, several authors have described laparoscopic modifications of the Vecchietti method in which the vesico-rectal septum is protruded with a thread-bearing cutting needle laparoscopically down to the vaginal groove, followed by placement of a vaginal dilatation instrument which is attached to the abdominal wall with preperitoneal traction sutures.
In summary, we have described several modifications of existing avidity techniques that have the potential to broaden the use of avidity assays in diagnosis and research.
Several recent studies have described modifications of immune function in aged subjects, leading either to increased, or more frequently decreased inflammatory processes with a reduction in LPS-induced IL-6, and IFNγ levels, similar to what we observed [30], [31], [32], [33].
