We have not yet been able to generate mice that have complete deletion of Pten in Nestin-positive cells.
While 40% of arm2/arm2; Dcas1/Dcas1 flies retained the lawn of denticles characteristic of an arm2/arm2 mutant (compare Fig. 7C, panels iv and vi), 34% had complete deletion of ventral and dorsal denticle belts (Fig. 7C, panel vii), while 26% had well-separated posterior denticle belts (Fig. 7C, panel viii).
Sixty-seven accessions had complete deletions of the three tightly linked ZCCT-H genes at VRN-H2.
Eighteen vernalization-insensitive genotypes contained just the c form of the ZCCT-H gene family but had complete deletions of the a and b forms.
To further confirm that the lethality seen with homozygous Top2c and Top2f is due to the loss of Topo II function, we placed Top2c and Top2f over the deficiency allele Df 2L Exel9043 that has a complete deletion of the Top2 gene and displays lethality during the first instar larva stage.
For example, in Canada, a predictably severe patient (i.e., has a complete deletion of the gene) under the age of 5 years would likely be treated, but an older patient who presents clear evidence of neurodegeneration would not (Lorne Clarke, personal communication).
This suggests that Bd21 resembles the vrn-2 mutant of spring barley in having a complete deletion of the gene and that a VRN-2 orthologue will be present in Brachypodium accessions with a vernalization requirement.
The last one had a complete deletion of this gene, a mechanism rarely found associated with clinical INH-resistance [ 26, 27].
Moreover, our data is consistent with pan genome analysis of seven wild soybean accessions (G. soja A to G) accessions [ 18] in which only G. soja D from Yellow River basin (Huanghuai) had a complete deletion of mature miR1511.
