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We demonstrate that LovD has broad substrate specificity towards the acyl carrier, the acyl substrate, and the decalin acyl acceptor.
Moreover, the AcCR has broad substrate specificity to a range of ketones and ketoesters, and could catalyze to produce chiral alcohol with e.e. >99% for the majority of tested substrates following the anti-Prelog rule.
Of these enzymes, Sulfolobus solfataricus β-glycosidase (SS-BGL) is the most efficient enzyme for the production of compound K from ginseng extract because it has broad substrate specificity and high activity for ginsenosides (Noh et al. 2009; Shin et al. 2015).
This cobalamin reductase has broad substrate specificity and is able to reduce a number of corrin based substrates from Co II) to Co(I) by a single electron reduction [6].
Neutral endopeptidase 24.11 (NEP, neprilysin, CD10) is a zinc metallopeptidase that has broad substrate specificity and cleaves several important peptides, including bombesin, ET-1, and the Alzheimer-associated amyloid β-peptide, thereby regulating their turnover and physiological and pathophysiological activities [1], [2], [3], [4].
This group of transporters has broad substrate specificity.
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Vaccinia virus thymidylate kinase, although similar in sequence to human TMP kinase, has broader substrate specificity and phosphorylates (E -5- 2-bromovinyl -dUMP and dGMP.
MMP-3 has broader substrate specificity; ADAMTS-4 and -5 cleave proteoglycans [ 30].
It has broader substrate specificity with activity against type II, III, IV, IX, X, XI collagens, proteoglycans, fibronectin and laminin.
Heparinase II has broader substrate specificity cleaving a wide range of disaccharide repeat units with either 2- O-sulfo or 2-OH at the uronic acid [ 4].
This study provides further evidence that S. strictum GOOX has broader substrate specificity than the enzyme name implies, and that substrate inhibition can be reduced by removing aromatic side chains in the -2 binding subsite.
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