The suppression of recombination in the region of the inversion fixates gene combinations that otherwise might have been unlinked by meiotic recombination.
No linkage disequilibrium among loci has been detected in any species suggesting that they are unlinked in A. nerii as well [27], [28].
This is particularly relevant as Golgi ribbon unlinking has been proposed to act as a checkpoint for cell entry into mitosis and is driven in part by phosphorylation of both GRASP65 and 55 during G2 phase (Duran et al., 2008; Feinstein and Linstedt, 2008; Persico et al., 2009; Rabouille and Kondylis, 2007; Sütterlin et al., 2002).
Re-diploidization has been subsequently characterized as a gradual process unlinked to significant genome rearrangements (Berthelot et al. 2014).
The level of incidence overestimation by unlinked HDD has been estimated to be between 7% 31% by one study which directly compared unlinked HDD with person-identifying (linked) HDD [ 10].
This ruling has been publicly dubbed a 'right to be forgotten', although that's a misleading descriptor — since the right being afforded is only for private individuals to unlink outdated or irrelevant information from a specific search for their name.
The guidelines do not require the consent of the donor if the samples have been anonymised and unlinked, but rather the consent of the Gambia Government/MRC Laboratories Joint Ethics Committee (the Ethics Committee).
However, multiple inserted copies (either linked or unlinked) have been implicated in issues with transgene silencing [ 5] and are not suitable for lines being considered for deregulation, indicating a need to identify linked copies.
Furthermore the samples that we used had been discarded following routine diagnostic analysis, and they were unlinked and anonymised so as to permanently protect patient confidentiality.
Another striking feature was that once parameters for modeling rate variation had been incorporated into the model, unlinking topologies did not seem to have as pronounced effect on the model likelihood (Table 2), compared to the models without rate variation.
However, the role of membrane remodeling during bacterial infection has not been determined and it is unlinked to the disruption of tight junctions (TJ), NHE3 inhibition (see below), or antiphagocytosis [ 114].
