This line has been selected by FACS-sorting of GFP-expressing parasites and therefore does not contain a drug-selectable marker.
In the present study exploiting Cre/ lox mediated recombination strategy, attempt has been made for selectable marker gene elimination from Allium sativum leaf agglutinin (ASAL) expressing Brassica plants with hemipteran insect resistant phenotype.
Most commonly, a single point mutation in a histidine residue, located in the third cysteine-rich cluster of the SDHB subunit (gene name: sdhb or sdi1 or Ip) has been used as selectable marker in fungi.
The most promising candidate for a selectable marker gene in Pandorina was the aphVIII gene of Streptomyces rimosus that has been used as a selectable marker in C. reinhardtii[ 25], V. carteri[ 30, 31], G. pectorale[ 20] and E. elegans[ 11].
The resistance provided by BlmB has been used as a selectable marker in bacteria, fungi, and mammalian cells.
Using frequencies of some of the data collected during this pilot phase, it has been possible to provide selectable options for future CABSYNC forms instead of open string fields.
Finally, the antigen CD15, also known as LeX or stage-specific embryonic antigen 1 (SSEA1), has been identified as a positive selectable marker for rodent multipotent NSCs [23].
Plasmid pPmr3 with the Streptomyces rimosus aphVIII selectable marker gene has been described previously [GenBank: AY429514] [ 8, 40- 42].
Although transgenic crop adoption has been increasing, the fact that a selectable marker gene (SMG, usually an antibiotic- or herbicide-resistant gene [ 7]) remains in the genomes of GM plants has raised concerns from both regulatory agencies and the public in certain countries.
