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Consistent with standard protocols, uniaxial accelerometer data were processed for analysis (see below for details).
Sections were processed for immunofluorescence as described below.
The brains were processed for immunohistochemistry as described below.
The brain slices were processed for immunohistochemistry as described below.
Human disc tissues were processed undecalcified and embedded in paraffin, and were processed for immunohistochemistry as described below.
Snap frozen fresh tissue cores procured as described below were processed for RNA and histology [27].
Alternatively, cells were processed for immunofluorescence staining as described below.
Following incubation the cells were processed for western blotting analysis and apoptosis detection as described below.
Fibrin gels were processed for histology, immunofluorescence, biochemistry, biomechanical or gene expression analysis as described below.
A slow dissolution process has been evidenced at potentials slightly below 0.80 V for the NO adlayer in alkaline solutions.
