PCR has been indispensible for gene cloning because it can provide target gene fragments in vitro in large amount and short time without the limitation of restriction sites.
Chromosomal knobs have been indispensible tools in the study of maize genetics for many years (Creighton and McClintock 1931).
Sequencing studies and characterization of the epigenetic landscape in lymphomas, leukemias and in aged healthy individuals with clonal hematopoiesis have been indispensible to identify epigenetic regulators that play a role in transformation or pre-disposition to hematopoietic malignancies.
But the broader lesson that one has to think critically about the counterfactual in trying to assess technologies or shocks or economic policy—has been an indispensible one for me.(Photo credit: AFP).
Since 1949, the ice resurfacer has been an indispensible part of rinks across the country; offering a slice of calm amidst the frenzy of a hockey game.
In all instances thus far examined myristoylation of the Ca2+-sensor and hence correct sub-cellular targeting has been proven indispensible to protein function [9], [11].
Mitofusin 2 (Mfn2; an OMM GTPase primarily responsible for mitochondrial fusion), is phosphorylated by PINK1 and has been shown to be indispensible for depolarization-induced Parkin translocation to mitochondria in cardiomyocytes (Chen and Dorn, 2013).
However, Rictor knockout mice showed no defects in adipogenesis (Kumar et al., 2010), although an mTORC2 substrate, BTSA (a BSD domain-containing protein) has been characterized to be indispensible for adipogenesis (Kumar et al., 2010).
In this regard, Continuing Medical Education (CME) has been propounded as an indispensible means for catching up with accelerating advances in health science and technology, as well as for improving medical performance, while financial resources allocated to it have been steadily increasing.
