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Each aligned read was assigned a mapping quality that indicates whether the read has a unique alignment or can be aligned to multiple positions in the genome [ 22].
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We considered a read to be evidence for an exon-exon junction if it had a unique alignment to a target with zero or one mismatch and no indels and if the alignment extended past the midpoint of the target by a certain overhang amount.
Consistent with this hypothesis the cyanobacterial sequences are well separated from the other sequences in the tree and have a unique alignment feature; they all share a ~160 aa highly conserved C-terminal extension that is absent from all other sequences.
A read is considered to be mapped if it has a unique best alignment.
We generated a total of 2400 alignments of each of the protein models available in Seq-Gen (100 alignments per model) consisting of 500 characters in length in this way so that each individual alignment has a unique set of amino acid frequencies.
Constrained by the topography of the site, the route of the Eastern Railway tracks, and the previous alignment of Great Eastern Highway, the bridge has a unique curved and sloping design.
Each of these elements has a unique ID and entry fields are typically auto-complete to ensure data alignment.
GensearchNGS has a unique approach to this problem by enabling the user to combine the various distribution methods to perform sequence alignment.
Gabarra has a unique perspective.
It has a unique history.
The Academy has a unique take.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com