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We also note that VP26 has a direct interaction with the nucleocapsid protein VP51C [30].
It is tempting to believe that ROCK1 has a direct interaction with Erk1/2.
Thioredoxin, linked to DR_1271, has a direct interaction with DNA polymerase.
IGHM has a direct interaction with amyloid-β precursor protein (APP), the latter in indirect interaction with IGF1.
It has a direct interaction with the DNA-repairing RAD23 superfamily of proteins that plays a central role both in proteosomal degradation of misfolded proteins and DNA repair.
ERα has a direct interaction with the CYP1A1 promoter, suggesting that it acts as a co-regulator of AhR-mediated transcriptional activation [ 60].
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The network contains two main hubs, AKT1 and GABBR1, which, although do not have a direct interaction, both interact with ATF2.
For example, mass spectrometry detects which proteins are part of a stable complex, but does not necessarily indicate which proteins in the complex have a direct interaction.
In the crystal structure a single residue, Asp533, is observed to have a direct interaction with the oxydril oxygen on topotecan ring E. In the simulation such an interaction is substituted by a water mediated contact, present for 84% of simulation time (see Figure 4 for a representative snapshot of the topotecan binding pocket in the simulation).
GST pull-down analysis showed ROCK1 had a direct interaction with GST-Erk2.
Also, asbestos could have a direct interaction with cellular constituents [ 7] such as the nucleosome and spliceosome complexes.
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