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Harvests of samples that induced cytopathic effect (CPE) in primary bovine thyroid (BTY) cells were tested in antigen ELISA (Ag-ELISA) and in 5'UTR-targeted real time RT-PCR (5'UTR RT-qPCR).
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Various methods are employed for measuring biomass/carbon include destructive (total harvesting of sample), nondestructive (measurable parameters like basal area, height, tree density), tree inventories data, species-specific biomass estimation, and remote sensing methods (Brown et al. 1989, 1999; Lu 2006; Murali et al. 2005).
Two independently grown and harvested sets of samples were used for RNA extraction and hybridization.
The conditions of the harvests (including the harvest hardware), storage of samples and the transport from the ISS to the researchers, were carefully replicated for each individual ground control.
The OD750 measurements of the harvest and of sample taken from the reactors were used to calculate the productivity of each photobioreactor.
For harvesting of CN samples, cells of 16 flasks were pooled.
The harvesting of the samples were possible in all patients in both groups.
The cold surface is used to keep the slices cold during the sectioning of the fresh, cerebral hemisphere, which then, during the harvest of the samples, will be frozen and banked.
In comparison to the lumbar vertebroplasty procedures in sheep, the femoral-tibial augmentation model proved to be safe, easy and quick to apply, and additionally showed a high level of standardization including the harvesting of core samples for histology and the histology evaluation.
Five plants per pot were grown at 10°C day/7°C night, 60% relative humidity, 12 h light:12 h darkness, 500 μmol m-2 s-1 photon flux density until the third leaf stage then 22°C day/18°C night, 55% relative humidity, 16 h light:8 h darkness 500 μmol m-2 s-1 photon flux density until harvesting of the samples.
DON was found in 73.08% of samples harvested in 2010 year, with highest levels of contamination 3.39 ppm.
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