Primer sequences for genotyping transgenic animals (by collection of DNA harvested from tail biopsies) span the Brk coding sequence (sense, 5'-agcgtgcacaagctgatgct-3') and the bovine growth hormone poly-A region of the transgene (antisense, 5'-tctctggctgtctgtctgca-3').
For the detection of rotenone in plasma, 200 µl of blood was harvested from the tail vein every week and the plasma was kept frozen at −80°C until use.
Mice were weighed, then lightly anesthetized with inhaled isoflurane; a drop of blood was harvested from the tail or submandibular vein.
Immediately after sacrifice, 0.5-g sections of tail were harvested for histological analysis and RNA extraction.
After sacrifice, 500 mg sections of the tail were harvested for paraffin embedding and RNA extraction.
We subsequently established MDA-MB-435-GFP sub-lines (MDA-MB-435-GFP-L) from pleural metastases harvested 12 weeks after tail vein injection of MDA-MB-435-GFP cells.
The tip of each fetus's tail was harvested for genotyping by PCR, after which the fetus and placenta were fixed by immersion in either 4% PFA, 10% NBF, or Bouin's solution.
MOPC315.BM cells were cultured in RPMI with 10% fetal calf serum in vitro (37 °C, 5% CO2) and harvested for injection into tail veins.
Following skin transplantation, the engrafted skin graft from the same donor tail was harvested at indicated time points (1~2 weeks and >1 month).
Selected organs (tumor, heart, lung, spleen, pancreas, stomach, small intestine, large intestine, kidney, liver, muscle, bone, and tail) were harvested, weighed, and counted using a WIZARD automatic γ-counter from PerkinElmer.
