Sentence examples for harvesting step from inspiring English sources

In addition, chondroprogenitor cells were capable of undergoing bead-to-bead migration, which allowed subcultivation to be performed without a harvesting step, thus improving the scalability of the expansion process.

Nevertheless, harvesting step of biomass is still a critical challenge.

These include scalability of culture in a cost effective manner [ 9], and the potential ability to deliver cells post-expansion without a cell harvesting step on biodegradable microcarriers [ 11, 12] for selected applications such as cartilage and bone healing.

Thus, for example, inhibitors of HK, the first glycolytic enzyme that phosphorylates glucose to produce glucose-6-phosphate, interfere with the preparatory phase, while inhibitors of the last glycolytic enzyme LDHA, which catalyzes the reversible conversion reaction of pyruvate to lactate coupled with the recycling of NAD+, abolish the glycolytic harvesting step.

The culture was then incubated to an OD600 of 1.0, after which cells were harvested by centrifugation (3,000 × g for 20 min) and washed by first resuspending in 10 mL of HSM (pre-warmed to 34°C) and then repeating the harvesting step.

Recycling of the supernatant was shown to be possible but should be repeated in a long term experiment comprising several harvesting steps.

Among the various steps entailed in the production of biodiesel from microalgae, the efficiency and cost-reduction of the cultivation and harvesting steps remain key obstacles to its practical commercialization.

For each time point in the aging experiment, three samples (mixed-cell samples 1,2,3; originating from different harvesting steps) composed of different fractions of Mother (M, green), Daughter (D, blue) and Dead cells (De, red) were harvested and analyzed.

To stabilize physiologically relevant interactions, adherent cells were crosslinked by a short treatment with formaldehyde prior to the cell harvest step [17].

This novel and efficient harvest step can be easily integrated into current mAb production platforms.

The application of flocculation using a stimulus responsive polymer, SmP E, enables a robust alternative cell culture harvest step which may ameliorate bottlenecks in downstream processes, particularly for challenging monoclonal antibodies.