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For nodule harvesting, segments of nodulated root systems were kept on ice for a few minutes while nodules were cut out; those were immediately frozen in liquid nitrogen and stored at −80°C.
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For larger-scale fishing, the government sells two-year leases to harvest segments of the Tonle Sap and inland rivers.
Subdivision of the vineyard based on remote sensing characterization allowed Mondavi to harvest segments of its fields at different times to coincide better with optimal ripeness.
To facilitate comparison of our data with the results of other transcriptome studies in inflorescence stems, we also harvested a segment (termed 'mature', or OLD) from the base of each of the stems and pooled these for inclusion in our transcript profiling analysis.
The increased expression of PAI-1 was seen in segments harvested from subjects with or without preceding insulin-resistant states.
Each sample was a pool of 10 segments harvested from the central 2 cm of each internode.
Following lumbar segment harvesting, the muscle tissues, intertransverse ligaments, and supra- and interspinous ligaments were carefully dissected, leaving the posterior joints and the IVD intact.
In order to position transcript profiles accurately within the cell wall expansion continuum, we employed growth kinematic profiling to establish relative elemental growth rates (REGRs) for contiguous stem segments harvested from a series of individual plants [ 15].
Aortic segments harvested from CBA.Ca mice were transplanted into fully allogeneic C57BL/6 wild-type (wt) or ApoE−/− recipients by an end-to-end infrarenal interposition technique as previously described [16].
To evaluate activated GSK-3 β signals in the avulsed spinal cord, we directly stained 30 μm cross-sections of fixed C7 segments harvested from the remaining animals with the antibody against p-GSK-3 βTyr216.
Distal nerve segments harvested for morphometric analysis at +10.5 mm to the proximal coaptation site were fixed in a fixative according to Karnovsky (2% paraformaldehyde, 2.5% glutaraldehyde in 0.2 M sodium cacodylate buffer, pH 7.3) for 24 hours and rinsed three times with 0.1 M potassium dichromate containing 7.5% sucrose before postfixation in 1% OsO4 was performed for 1.5 hours [ 30, 32, 37].
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