Several factors influence flavonoid synthesis in herbs and crops, such as environmental conditions (light intensity, CO2 concentration, temperature) [27 30], and agricultural practices (fertilizer, irrigation, harvesting, post-harvesting) [31 34].
Tertiary bioresources may be generated along the chain in primary bioresource production, harvesting, post-harvesting and storage activities, and during industrial processing, packaging and distribution up to the retail sector.
Depending on the time at which RCM is harvested post irradiation, the activated TGF- β1 pathway can either upregulate or downregulate miR-21 expression in bystander cells.
Cells were harvested post-incubation by trypsinization and washed twice with PBS.
The panicles from WT and mutant lines were harvested post-maturity (40 DAH) and air-dried for 2 weeks before analyses.
Mammary glands and tumors were harvested post-mortem, snap-frozen in liquid nitrogen, and stored at −80°C.
Mammary glands and tumors were harvested post-mortem and stained with X-gal to detect β-gal activity, as previously described [72].
Brains were harvested, post-fixed overnight in 4% PFA, then cryoprotected in 30% sucrose-TBS.
Cells were harvested post-stimulation, RNA prepared and cDNA synthesised as described in Section 2.2.
Cells were harvested post-ADI-PEG20 at days 2 4 with cell line counting performed using a Beckman Vi-Cell cell viability analyser (Beckman Coulter Ltd ,High Wycombe, UK).
Radiotherapy before surgery may explain the lower proportion in rectal cancer patients due to reduced lymph node harvests post-radiotherapy [ 24, 25].
