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FLuc activity in cell lysates was quantified by extracting cells from Matrigel using a 3D cell harvesting kit (Trevigen).
3-D colonies were harvested with Cultrex 3D culture Matrix™Cell Harvesting Kit (3448-020-K, TreviGaithersburgsburg, MD, USA) following the manufacturer's instruction and total RNA isolated with Qiagen RNeasy Plus Mini kit (#74134).
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Many of the fair's longtime commercial exhibitors, manufacturers of solar-energy technology or rainwater harvesting kits, could now find a home at the Home Depot.
In the latter, well, how else to pay for all those expensive new barrels, whizzy harvest kit such as digital grape-sorting tables and experienced staff?
Chronic type II (>1.5 cm2, <5 mm deep), mean size 2.07 +/− 0.48 cmeanmean depth 4.0 +/− 0.9 mm Giannini et al. 2013 N = 20 Not Reported 36 MFC (14), LFC (4), None Reported 60 mL of bone marrow harvested from the posterior iliac crest and concentrated with a Harvest Tech Smart PReP using the Harvest BMAC kit to obtain 6 mL of BMC.
The isolation of nuclei was performed on fresh plant material (fronds) immediately after harvesting using the Cystain PI Absolute P kit (Partec).
Periplasmic and cytoplasmic fractions were prepared from cell lysate from the harvest samples using PeriPreps™Periplasting kit (Epicentre, Madison, WI, USA).
RNA for microarrays was harvested using the RNeasy kit.
RNA from differentiating P19 EC cells was harvested using RNeasy Mini Kit (Qiagen, Canada) and analyzed using real-time quantitative PCR (QPCR) as described in [48], [50].
RNA was harvested using a microRneasy Kit (Quaigen).
Total RNA was harvested using the RNeasy Kit (Qiagen, Valencia, CA, USA).
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