Clausen Oysters, a locally owned company, sued Green Atlas Shipping over oil-damaged harvesting grounds.
Similarly, Sightglass Coffee (@sightglass) shares photos from their harvesting grounds in El Salvador and Ethiopia to remind us that even a local business has global connections.
Although Anderson sympathized with the landowners, who wished to make their land productive and protect themselves from litigation by anyone who was injured on their ranch while collecting peyote, the closure of peyote harvesting grounds produced "serious tensions" between indigenous people and the ranchers.
The oats have been harvested, ground up, reprocessed and turned into soggy cornflakes.
The plant material (on average 0.8 to 1.0 g) was then harvested, ground in liquid nitrogen and extracted twice with 5 ml aqueous methanol (50+50, v+v).
Samples were frozen in liquid nitrogen immediately after harvest, ground with pre-chilled mortar and pestle, and stored at -80°C before use.
Four disks (1 cm) were harvested, ground in 10 mM MgCl2, and plated on KB with rifampicin and cycloheximide on days 0 and 3 for colony counting.
Briefly, the mycelia were harvested, ground under liquid nitrogen, mixed with extraction buffer and centrifuged at 14,000 g at 4°C for 10 min. The supernatant was recovered and used for the experiments.
Approximately 50 mg seedlings were harvested, ground in liquid nitrogen and resuspended using 100 μl extraction buffer (50 mM HEPES-KOH pH 7.9, 400 mM KCl, 2.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1 % Triton X-100) supplemented with EDTA-free protease inhibitor cocktail (Roche, Vienna, Austria).
Significant decline in oyster population at Cedar Point Reef, the primary oyster harvest grounds in Mobile Bay, had persisted since then until the Cut was artificially closed in 2010.
