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To determine whether the induced increase in α7-nAChR protein levels in the membrane compartment reflects an upregulation of receptor protein at the plasma membrane, peptide-treated GH4-hα7 cells were exposed to the cross-linking agent BS3 prior to harvesting for analysis.
Cells were incubated for 48 h before harvesting for analysis using flow cytometry.
These RNAi plates were allowed to dry overnight and stored at 4°. Animals were placed onto RNAi bacteria as embryos and grown for approximately 48 hours at 20° before harvesting for analysis by immunoblot.
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Cells were harvested for analysis 84 h after transfection.
Plasma, liver, and epididymal fat pad samples were harvested for analysis.
At 48 h post-transfection, protein and RNA were harvested for analysis.
In addition, aortas were harvested for analysis of atherosclerotic lesions.
After a further 2 weeks of in vitro culture, pellets were harvested for analysis.
Nonwounded fetal skin at E17, E19, and E21 was harvested for analysis of decorin expression during skin development and as controls for wounds.
One-week-old seedlings were harvest for analysis.
On days 15 17, the animals were euthanized; leg bones and visceral metastases were harvested for analysis.
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