[methyl-H]-Thymidine (20 μCi/mL) (PerkinElmer, Waltham, MA, USA) was added 24 hours before harvesting cells on fiberglass filters by means of an automated cell harvester (PerkinElmer).
The process of harvesting cells from the dermal-epidermal junction by enzymatic and physical dissociation is used for immediate delivery of a noncultured cell population to the wound.
Olfactory ensheathing glia were found in the mucosa at both these sites implying that surgical access for harvesting cells for transplantation would be straightforward.
The determination of a suitable culture age during growth of P. aeruginosa in medium for maximum product formation was accomplished by harvesting cells in various stages of growth and employing them for myrcene biotransformation.
The microbial biomass was determined by harvesting cells by centrifugation, drying them at 70°C under vacuum to a constant weight, and expressed as gram dry cell weight (DCW) per liter [27].
T cell activation was monitored by harvesting cells from 96 well plates and subjected to FACS analysis (FACSCanto II, BD Biosciences, San Jose, CA) after staining with anti-CD4, CD8, CD25, and CD69 fluorescently-labeled antibodies (eBioscience, San Diego, CA).
After harvesting cells, GST fusion proteins of PLD1 were purified as previously described [41].
Growth yields were calculated by harvesting cells by centrifugation from 40 ml broth at the beginning of stationary phase.
For the oligomycin experiments, cells were treated with 1 µg/ml of oligomycin for 30 minutes prior to harvesting cells.
The time of harvesting cells was set at early exponential phase, middle exponential phase, late exponential phase, and stationary phase.
To assess the production of PIA, overnight cultures were normalized with respect to each other before harvesting cells by centrifugation.
