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Vessels 100 300 μM in diameter were dissected from the harvested section of left ventricle.
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Cells were harvested, sectioned and lysed in Evan's protein lysis buffer (1 mM EDTA, 1 mM EGTA, 1%NP-40, 50 mM Tris-HCl, 120 nM NaCl and peptide inhibitors).
Then the uterus was harvested, sectioned and incubated with FITC-conjugated anti-hypoxyprobe antibody.
Hind limbs were harvested, sectioned and evaluated for DMARD activity and general histopathology by histomorphometric analysis and immunohistochemistry (vWF staining).
Organs were harvested, sections were taken for histological analysis and the remainder were snap-frozen in liquid nitrogen.
The animals were then sacrificed at 7, 10, and 14 days post AM9D injection, and mammary tumors were harvested, sectioned, and viewed under a fluorescent microscope.
Liver and lung tissues were harvested, sectioned and then stained with hematoxylin and eosin (HE) for histological analyses to observe secondary metastasis.
Mice were euthanized and brains were harvested and sectioned into 1.6-mm coronal sections (5 sections) and stored at −80 °C.
After tumors were harvested and sectioned, MNPs in tumor sections and other tissues were identified as Prussian blue positive material in tumor bearing mice intravenously injected with MNPs.
Although it is a regular chain supermarket, Star offers a rather large selection of health/vegetarian foods in its Wild Harvest section.
The control variables in the thinning and harvesting section all determine the speed of conversion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com