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After mammographic identification of the lesion, CNBs are harvested, placed in tissue trays, and x-rayed to confirm sampling of the lesion.
Pseudo-nitzschia fraudulenta cells were harvested, placed in a µ-dish (Ibidi) and stained for 60 minutes with Oregon Green BAPTA-1 AM (Thermo Fisher) at 10 µM with a final concentration of 0.1% DMSO and observed immediately using a DeltaVision System.
After 20 h, mice were sacrificed and tumors were harvested, placed in a dry ice/2-butane bath and embedded in optimal cutting temperature solution.
At 14 days after the fifth spray, the available unopened flower stems were harvested, placed in buckets containing distilled water, and were immediately transferred to a greenhouse of the Horticulture Department at Gyeongsang National University, Jinju, South Korea.
Sacrified females (human end point) and 3 days after weaning pregnant females were euthanized using CO2, and mammary glands were harvested, placed on dry, silanized glass slides and fixed overnight in 1∶3∶6 parts of glacial acetic acid∶chloroform∶100% ethanol.
Mouse RPE and choroid were harvested, placed in 10% RIPA buffer (Sigma-Aldrich) and sonicated 3 5 times.
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These would be harvested and placed into plastic "stomachs" – essentially large plastic bags – suspended in the sea.
Six heads from each plot were harvested and placed in 4 °C forced-air cooled room for 1 day before processing.
For ex vivo RAD51 foci assay, tumor tissue was first harvested, then placed in cell culturing medium and shortly after irradiated with 10 Gy or left untreated.
Algal cells at different growth phases were cultivated in glass bubbling columns for 7, 14, 21 and 28 days, harvested and placed in the attached photobioreactors.
Zygospores were harvested and placed on wet filter paper.
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