Harvesting and screening for rhizosheath and RSA characteristics were done 7 days after sowing.
After three days, F1 with corresponding F2 progeny were harvested and screened by PCR amplification.
T0 seeds were harvested and screened by germinating on MS solid medium with antibiotic selection.
For the second EMS mutagenesis treatment, ∼5500 BC2F4 dmseedsseeds were treated as described above, and M2 seeds from ∼3575 M1 plants were harvested and screened for GFP.
Cells showing CPE were harvested and screened by the Syva MicroTrak HSV-1 and HSV-2 culture confirmation system (Behring, Milton Keynes, England).
Leaves, stems, various stages of fruits (early, mid, turning and late), roots and flowers from mature plants (3 months old) were harvested for screening tissue-specific CanPIs.
Fourteen days after in vitro stimulation, ex vivo expanded V γ9V δ2 T cells were harvested and screened for their sterility (negative for endotoxin, bacteria, fungus, and mycoplasma contamination) and purity (>60%).
This current study was undertaken to determine whether cross-neutralization of other HPV types is similarly dependent on time of harvest and to screen for the most effective cross-neutralizing epitope in native virions.
At the moment, even asking fairly straightforward questions requires a biologist to spend hours in front of a screen, harvesting databases and finding the tools needed to gather evidence.
The film premieres in New York City on July 29 at the Quad Cinema and Los Angeles on August 5 at the Laemmle's Music Hall 3. Additionally, The Harvest will be screened in up to 30 cities nationally following the theatrical release.
