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Except in a few cases, soil microbial respiration, biomass and activity were higher under zero tillage than under reduced tillage, both at planting and harvest sampling times.
Seed for each generation were drawn at random from the previous harvest and cumulative probability of extinction through stochastic harvest sampling was calculated at each generation.
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Periplasmic and cytoplasmic fractions were prepared from cell lysate from the harvest samples using PeriPreps™Periplasting kit (Epicentre, Madison, WI, USA).
Prior to harvest, samples were washed twice in PBS pH 7.5.
Immediately following tissue harvest, samples for histology were fixed in 10% formalin.
Harvest samples were froze immediately in liquid nitrogen, and stored at 80°C.
Along the developmental stage dimension we chose harvest samples through Phase 1 ripening (147, 168, 175, 224 and 231 DAFB).
Linearly spaced harvesting in time would cause increasing information overlap between neighboring time points, thus it was decided to harvest samples exponentially spaced in time.
Thus, each brain donated to the NYBB is an opportunity to establish definitive postmortem diagnoses, teach trainees, and harvest samples for research.
At the time of harvest, samples were preserved in 10% formalin and sent for micro-CT scans and then histologic staining.
In general, it is appropriate to harvest samples at entry into the study, as well as 2 weeks and 4 weeks after treatment.
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