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The annotations of the Barley1 GeneChip were updated by the HarvEST database [ 52].
The Probesets were annotated by searching against the Arabidopsis genome using the tool provided in HarvEST database (http://harvest-web.org).
For this analysis we used the HarvEST database which contains over 50,000 unigenes and searched for miRNA precursors corresponding to smRNA sequences in our database.
In order to approach this large genome, several projects have generated significant numbers of expressed sequence tags (ESTs) (ca. 500 000) (see HarvEST database http://harvest.ucr.edu/).ucr.edu/
We also compared the new citrus ESTs with the ESTs and unigenes already available in the Citrus HarvEST database, which contains 229,570 ESTs.
Finally, the new citrus ESTs collection added almost 2000 new genes not previously represented in the Citrus HarvEST database, which already contains 229,570 ESTs.
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The first step toward developing the high throughput platform was to curate all known hordeins sequences from EMBL Nucleotide Sequence Database (EMBL), DNA Data Bank of Japan (DDBJ), GenBank at the NCBI (GenBank) and HarvEST databases.
At the moment, even asking fairly straightforward questions requires a biologist to spend hours in front of a screen, harvesting databases and finding the tools needed to gather evidence.
The array included 301 probes derived from a subtracted SSH library, a cDNA AFLP collection, and a set of regulatory genes from the Harvest citrus database.
Probe set information was retrieved from Harvest: Citrus database http://harvest.ucr.edu, http://www.harvest-web.org.
Quality controlled EST information is available from the HarvEST:Barley database [ 3].
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