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In recent years, several activities aiming at the harmonization of techniques used to monitor the presence of antigen-specific T-cells have been initiated for ELISPOT [ 28– 31], tetramer staining [ 32] and ICS [ 33– 33].
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In this regard we take note of the work of the environmental experts on the improvement and harmonization of the techniques and practices of environmental measurement and ask them to report as soon as possible.
Few attempts to unify the monitoring methodology have been made (e.g., guidelines developed for HELCOM COMBINE program in 1999), but so far no real intercalibration or harmonization of those techniques has been carried out.
Such "two-step" inter-laboratory projects could define rational bases for accepted international guidelines and thereby lead to the harmonization of the techniques used for immune monitoring.
These results emphasize the need to identify the critical variables important for the observed variability to allow for harmonization of the technique across institutions.
One Finnish doctor spent 1 year in Denmark allowing a close harmonization of the examination techniques.
The substantial progress on "harmonization" of HbA1c measurement techniques across technologies, manufacturers, and individual laboratories around the globe that David Sacks describes in this issue of Diabetes Care (34) is another key step toward better understanding.
Improvements should be made in order to obtain better data, and there should be more harmonization of the data collection techniques to establish a reliable epidemiological database.
Our results emphasize the urgent need for harmonization of the multimer labeling technique.
Analytical techniques: International harmonization of methods for bioaerosol exposure assessment is needed.
The CIMT Immunoguiding Program CIPP) is one of a number of recent international initiatives that is committed to quality assurance and harmonization of the most commonly used techniques for cellular immunomonitoring, such as staining with HLA-peptide multimers, flow cytometric determination of intracellular cytokines and the IFN-γ ELISPOT assay [ 4– 7].
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