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The post harmonization algorithms are described in detail in harmonization guidelines attached to this paper.
Post harmonization algorithms are developed by finding a "common denominator" to merge the datasets and weights are calculated to adjust for differences in age and sex distribution across the datasets.
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Across the cohorts, different measurement instruments were used and post harmonization algorithms were needed to merge the datasets and enable statistical analyses that allow testing cross-country differences.
The basic concepts underlying the "codon harmonization" algorithm are derived from evidence provided in Thanaraj, et al, where codon usage frequency patterns from E. coli proteins having known structures were analyzed [22].
1. Site-directed mutagenesis for FMP003: The codon harmonization algorithm was applied to the gene fragment encoding MSP142 from the FVO strain of P. falciparum (GenBank accession no. L20092).
The algorithms are complex.
Algorithms aren't monsters.
Algorithms aren't magic.
Algorithms are involved.
The algorithm was further tested by preparing a "fully harmonized" MSP142 (FVO) gene fragment (designated FMP010), which was completely recoded according to the harmonization approach described in "Materials and Methods".
We have co-analysed data across the participating studies by applying careful harmonization algorithms.
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