Sentence examples for harmonization algorithm from inspiring English sources

The details of using the Codon Harmonization Algorithm to generate a recommended sequence for the FMP003 gene are given in Table S1.

2. Full codon harmonization of MSP142 FVO gene: The synthetic gene sequence for the MSP142 FVO synthetic gene fragment or FMP010 (GenBank Accession no. DQ926900) was developed by applying the codon harmonization algorithm over the entire gene sequence.

1. Site-directed mutagenesis for FMP003: The codon harmonization algorithm was applied to the gene fragment encoding MSP142 from the FVO strain of P. falciparum (GenBank accession no. L20092).

The basic concepts underlying the "codon harmonization" algorithm are derived from evidence provided in Thanaraj, et al, where codon usage frequency patterns from E. coli proteins having known structures were analyzed [22].

As described below, our initial studies evaluated the utility of this codon harmonization algorithm by focusing on this region of the gene because, of all of the putative regions of slowly translated mRNA that were identified, this was the most discordant in its codon usage pattern.

For expression, the plasmid was used to transform the BL21-DE3 expression host (Novagen, Milwaukee, WI). 3. Full codon harmonization of the MSP142 3D7.2 gene fragment: The sequence for the synthetic MSP142 3D7.2 gene fragment (GenBank Accession no. DQ926901) was developed by applying the full codon harmonization algorithm.

The post harmonization algorithms are described in detail in harmonization guidelines attached to this paper.

We have co-analysed data across the participating studies by applying careful harmonization algorithms.

Across the cohorts, different measurement instruments were used and post harmonization algorithms were needed to merge the datasets and enable statistical analyses that allow testing cross-country differences.

Post harmonization algorithms are developed by finding a "common denominator" to merge the datasets and weights are calculated to adjust for differences in age and sex distribution across the datasets.

A 522 bp DNA fragment of PfCelTOS 3D7 (Genbank Accession number AB194052) was designed by using the codon-harmonization algorithm [9].