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"It's very important especially with a hard sequence of games leading up to Australia and no doubt Wales will pick up injuries," Lewis said.
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The hard sequences usually consist of polybutylene terephthalate (PBT), a stiff polyester resin.
Other techniques were used by MWG Biotech (Eurofins) to resolve hard sequencing stops to complete the reads.
"It was the hardest sequence in the movie to decide on because we knew we wanted to do it from the get-go," he said.
The scene where Tapping had to strip and be hosed down with water was the hardest sequence Tapping had filmed in her entire acting career; Tapping "bawled [her] eyes out" between takes.
He had made his name in bouldering, very short unroped problems – no longer than 30ft – where the emphasis is on climbing the hardest sequences of moves, unencumbered by ropes and climbing gear.
In the AB-Medium and AB-Hard sequences it is also shown the robustness of the proposed system against occlusions in crowded scenes.
The false detections in the AB-Medium and AB-Hard sequences correspond in every case to persons staying static for a long time.
Advances in modern manufacturing systems such as CAD/CAM, FMS, CIM, have increased the use of intelligent techniques for solving various combinatorial and NP-hard sequencing and scheduling problems.
While we report some false detections in the AB-Medium and AB-Hard sequences, these detections do correspond in every case to static objects (persons staying static for a long time); we rated them as false detections, since they do not correspond to abandoned objects.
For templates that were hard to sequence, 1% (v/v) dimethyl sulfoxide was used in the sequencing reaction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com