Inside an anaerobic glove box the cell-free extract harboring the three [FeFe] hydrogenase helper proteins HydE, F, and G was reconstituted [26] by incubation at room temperature with 0.5 mM ferrous ammonium sulfate and 0.5 mM Na2S, and then spun at 10,000 g until all precipitates were removed.
However, MBS extract might harbor some advantages over ribavarin such as safety, low cost, and its natural origin which together allow using MBS extract, and/or the underlying antiviral component, in higher doses and longer periods than ribavarin; this ultimately leads to equal or superior therapeutic values of MBS extract to that of ribavarin.
Consistent with that, human extract produced eccDNA harboring mouse MSD from mouse genomic DNA with the same efficiency as the mouse extract.
After obtaining the domain partners, all the proteins harboring them were extracted using SwissPfam [ 44].
Lane a: crude extract of E. coli Rosetta2 (DE3) harboring pET-G1cscA (135.8 μg of protein), Lane b: purified recombinant protein (2 μg of protein), Lane c: crude extract of E. coli Rosetta2 (DE3) harboring empty vector (65.3 μg of protein), Lane M: molecular mass marker proteins.
Our results showed that the cell-free extract from recombinant E. coli JM109 harboring pBBR-CAB and pTrc-pct-bktB exhibited both Pct and β-ketothiolase activity.
To speed up VERSE, we extract genomic regions from host genomes that are likely to harbor virus integration sites.
A bearded man said that fluke, flounder, and harbor blues might be extracted from the river.
Today, PBMs enjoy a "safe harbor" from federal anti-trust rules and other regulations that allow them to extract these rebates from drug companies.
Although "State of Wonder" tackles the larger Hippocratic quandary posed by scientific exploration — how does one extract whatever raw materials one has come for without destroying the habitat and the indigenous people who harbor them?
For cultivation and induction of the E. coli BL21DE3 harboring the plasmid for the carbamoylase was lysogeny broth (LB) medium containing 10 g/L tryptone, 5 g/L yeast extract and 10 g/L NaCl.
