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Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline.
Here we describe a new procedure called BAC HAPPY MAPPING (BAP mapping) that is designed to overcome these limitations.
Six of the linkages by which scaffolds were extended have been independently confirmed by published HAPPY mapping links [ 35], and others have been confirmed by unpublished HAPPY results (E.O., E.P.H. and P. Dear).
Happy holidays, and happy mapping!
BAP mapping combines the principles of the BAC landing method [14] with HAPPY mapping [15], [16].
It is important for HAPPY mapping [15], that the BAC aliquots contain less than one haploid genome (0.6 0.7 fold coverage of the genome).
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The resulting F-maps were thresholded at significance level of p < 0.01, and cluster size being 16 for the anger maps and 15 for the happy maps.
The integrated HAPPY map has revealed the probable structure of the E. tenella genome, and explains why the ongoing sequencing program has encountered difficulties.
As a result of incorporating BAC-end and contig sequences, an integrated HAPPY map was constructed [Figure 1; Additional file 4].
As there are no HAPPY maps and genomic libraries with large inserts for C. hominis, the observations on genome organization of C. hominis need to be supported by PacBio sequencing.
As HAPPY maps for E. tenella chromosomes 1 and 2 have previously been constructed, we demonstrate in this study the construction of an integrated map for the remainder of the genome in order to give an overview of genome organization and to aid sequence assembly.
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