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Using three mapping panels of short-range, mid- and long-range, containing genomic fragments of 1.5, 2 and >2.5 Mbp, respectively, a HAPPY map consisting of 1001 STS markers spanning the entire 90 Mbp long arm of human chromosome 14 was constructed with a resolution of ∼100 Kbp [17].
However, the combination of BAC data with HAPPY map data is more robust.
Of these, 795 were considered as usable markers, and utilized in the construction of a HAPPY map.
BAC-end sequences and contigs from whole genome sequencing were also integrated to improve and validate the HAPPY map.
L-SL constructed the HAPPY map, and together with Y-LT, HA and PHD analyzed the data.
As a result of incorporating BAC-end and contig sequences, an integrated HAPPY map was constructed [Figure 1; Additional file 4].
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Happy holidays, and happy mapping!
BAP mapping combines the principles of the BAC landing method [14] with HAPPY mapping [15], [16].
It is to address this point that a second HAPPY mapping panel is created (Figure 1f), this time constructed using HMW fragments of genomic DNA.
Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline.
We demonstrate the value of BAP mapping for merging linkage and physical maps into one pipeline re-using 40 out of 107 markers from the FCA region of A. thaliana (that had been previously ordered by conventional HAPPY mapping, [24]) for BAP mapping.
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