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Most of the HAPPY linkages were supported by at least one BAC clone.
The BAC-end sequences also suggested that HAPPY mapping has correctly ordered the contigs, as most of the HAPPY linkages are supported by at least one BAC clone.
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Five new linkages (that is, linkages which had not already been made by HAPPY data alone) were identified by these clones, between the HAPPY linkage groups and large contigs that do not have any good markers.
The number of BAC clones that fall within these multi-copy contig groups often exceeds the number of clones within the HAPPY linkage group.
Two groups were found to have one of their end-markers originating from a contig that contains a good marker in one of the HAPPY linkage groups.
The 661 HAPPY markers which fall into linkage groups cover ~31.0 Mb of the genome, giving an average spacing on mapped regions of ~46.9 kb.
HAPPY mapping ([ 40]; a physical linkage method currently being used to join Tetrahymena scaffolds into complete MAC chromosomes) provides independent evidence that small scaffolds are enriched for MIC-limited DNA (Orias & Hamilton, unpublished observations alluded to in [ 35]).
If the respondent was happy to give consent to data linkage, they had to sign and date the consent form and tick the "yes" box next to the health data linkage text, see [ 10].
On the whole, YPs found the data linkage materials user-friendly and were happy to discuss this consent.
Six of the linkages by which scaffolds were extended have been independently confirmed by published HAPPY mapping links [ 35], and others have been confirmed by unpublished HAPPY results (E.O., E.P.H. and P. Dear).
NURSPH, Rwanda, respondents were extremely content with its links to MOH (5.0) and were happy in general with links to all other organizations mentioned above except for health facility linkages (2.0) and the media (1.3).
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