Equal volumes of protein solution and the reservoir solution containing 200 mM NaCl, 50 mM MgCl2, and 20% PEG3350 were mixed and were equilibrated in a hanging drop tray for 24 hr at 4°C.
Crystals were grown by vapor diffusion at 22°C by mixing 1 μl of sample with 1 μl of reservoir solution (20 mM MgCl2, 20 mM Tris HCl, pH 8.0, 1.6 M ammonium sulfate) in a 48-well hanging drop tray (Hampton Research, Aliso Viejo, CA, United States).
Crystals grew as plates from 17% PEG 3350, 0.1 M disodium phosphate in hanging drop trays.
We looked around the studio, surveying the older pieces -- the bowls and baskets and lids and trays hanging on hooks.
In addition there are the smaller details to account for, like the basket of lacquered vegetables (exactly what kind is a matter of backstage debate: zucchini, okra, bitter melon?), stacks of fake dollar bills, carts with hanging fish and trays of jewelry.
Crystals were set up in EasyXtal 15-well hanging drop crystal trays (Qiagen).
We've even run out of buns, which gives us a legitimate reason to feed hungry police officers hanging around the truck trays of pure bacon.
Use mirrored trays or hanging mirrors if you want your space to look larger.
Create a subdued version of this technique by hanging polished silver platters or trays on the wall.
Crystallization drops contained 1 μl RPo in 20 mM Tris HCl, pH 7.7, 100 mM NaCl, and 1% glycerol, and 1 μl reservoir buffer (RB; 100 mM Tris HCl, pH 8.4, 200 mM KCl, 50 mM MgCl2, and 9.5% PEG4000), and were equilibrated against 400 μl RB in a vapor-diffusion hanging-drop tray.
