Addition of FBS to hanging drop stage resulted in the appearance of beating cardiac clusters in some BMP-4 treated EBs.
In conclusion, BMP-4 is unable to induce cardiomyocyte differentiation from mouse ESCs in serum-free models, and at least small amount of FBS in hanging drop stage is necessary.
Jack Nicolson hanging back stage with The Monkees.
After the hanging drop stage, the embryoid bodies were then plated onto 0.1% gelatin-coated tissue culture dishes [2], [4], [44].
Cromer populates the stage with the Israeli locals, and has the Egyptian band members hanging around the stage providing background music, and yet we get the sense that each of these people have their own story, even if they never actually speak.
Grell, who was a manager at a button factory for much of his working life, became infatuated with Russian ballet, hanging out at stage doors to get autographs or spare toe shoes, and hurling voluminous quantities of flowers to stages during ovations.
A large fishing net hanging over the stage and a few salty props economically evoked the seaside.
This was everywhere: emblazoned across the main stage, hanging from banners and slipped in some guise into every minister's speech.
Mr. Johns's décor for it, based on Duchamp's "Large Glass," was a constant presence, hanging above the stage during the dancing.
