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The crystals formed in hanging drops over a well solution of 0.1 M HEPES, pH 7.5, 20 mM MnCl2, 0.1 M Na acetate, and 10% (w/v) PEG4000.
Crystals grew within 3 4 days at 20°C in hanging drops over a reservoir solution containing 10% PEG 8000, 100 mM Na/K Phosphate pH 6.2, 150 mM Guanidine hydrochloride, and 200 mM NaCl.
Crystallization of purified proteins was conducted using hanging drops over a 0.5-mL reservoir (15% w/v PEG8K, 200 mM Mg OAc 2, 100 mM 3- N-morpholino -2-hydroxypropanesulfonic acid (MOPSO)-Na+, pH 7.0).
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The analysis of the cells in hanging drops.
Average speed of HSC-3 cell movement in hanging drops.
We chose the hanging drops protocol as reviewed by Kurosawa [ 21].
BARD1 BRCT crystal form II CFIII) was grown by vapor diffusion in hanging drops at room temperature.
Within this time frame, cells coalescing at the bottom of the hanging drops formed sheets.
In addition, HUVECs (n = 3) were cultured as spheroids in hanging drops for 24 h.
The ratio precipitant:protein was 1 2 (v/v) in hanging drops of final volume 3 μl.
EBs were cultured in hanging drops for 3 days before being reseeded onto gelatin-coated plates.
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