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Data collection and handling were done with strict confidentiality.
All captures and handling were done on public lands with permits and the capture and handling procedures approved by the University of Saskatchewan's Committee on Animal Care and Supply (Permit Number: 20010016) following guidelines provided by the American Society of Mammalogists Animal Care and Use Committeee [ 66] and the Canadian Council on Animal Care [ 67] for the safe handling of wildlife.
All animal handling were done in compliance with the guidelines of the German Animal Welfare Act.
In addition, DNA extraction and PCR reagent handling were done in dedicated safety cabinets under sterile conditions.
All the sequence analyses and data handling were done using programs written in the Python programming language (ver. 2.6, http://www.python.org/).org/
Whole blood was sampled in trace element 6/7 mL Plus K2 EDTA tubes (368381) (BectonDickinson, NJ), and sampling and handling were done as described previously (Penny and Overgaard 2010), discarding the first sample.
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SAM/BAM and BED files handling was done using SAMtools and bedtools.
All fly handling was done at room temperature using CO2 anesthesia on flies not less than 6 h after eclosion.
Animals were daily identified and weighed, and handling was done in the same time range to avoid the influence of biological rhythms.
All fly handling was done at room temperature using CO2 anaesthesia only to sort virgin flies and to place females in the egg-laying chambers.
"While I am confident that all of the inquests handled were done so correctly, I apologise if this matter causes distress to the families and friends of the deceased.
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CEO of Professional Science Editing for Scientists @ prosciediting.com