The methods for sample collection and handling protocol as well as the laboratory work-up used in this study have previously been described in detail [ 4, 17].
Capture and use of animals as well as handling protocols were previously approved by the Ethics Committee on Use of Animals of the Federal University of Paraná under protocol number CEUA/SCA 057/2013.
The Beijing Friendship Hospital Animal Care Committee approved all animal handling protocols.
Furthermore, all animal handling protocols were approved by the Ethiopian Wildlife Conservation Authority.
This article provides an overview of the basic statistical approaches for analyzing clinical trials with binary, continuous or time-to-event outcomes as well as methods for handling protocol deviations due to noncompliance and early drop-out.
All animals were safely handled under animal handling protocol AUP R217-0509B (University of California, Berkeley).
The method of handling protocol deviations was described in 37 protocols and 43 publications.
†Among 32 trials that described methods of handling protocol deviations in both publication and protocol.
Furthermore, different sample handling and storage protocols as well as age of samples may have an effect not only on biomarker quality but also on biomarker levels (Table 2).
In addition, for the reproducibility of gut microbiota studies, standardization of protocols for animal handling as well as sample storage and processing and data analysis are of utmost importance.
