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The animal handling protocol of the present study was submitted to the institutional animal ethics committee, "Experimental Animal Ethics Committee, UODA" for evaluation and Animal experiment protocol was approved by the committee (approval Reference number: e/m-7PHA040294).
In this context, and because of the particular characteristics of this species (extensive exploitation typically far from laboratory facilities), a key goal is to optimize the design of an adequate handling protocol of sperm samples before samples are subjected to sex sorting and cryopreservation procedures to obtain better outputs from the application of these technologies.
Similar(58)
The classification of subsp. spontaneum as strong inbreeder (Abdel-Ghani et al. 2004) obviously resulted in handling protocols for genebanks (i.e., separation of different accessions by only few meters distance during multiplication, wild and domesticated cereals next to each other) that do not prevent hybridization.
The handling protocol included collection of blood, administration of ivermectin (0.2 mg/kg sc; Ivomec® 1%; Merial, Toulouse, France), checking of ear tags and microchips and an external clinical examination.
39 Previous reviews of high impact journals have found that a high proportion of publications provide insufficient details about methods of handling protocol deviations or missing data.
The method of handling protocol deviations was described in 37 protocols and 43 publications.
†Among 32 trials that described methods of handling protocol deviations in both publication and protocol.
*Among 43 trials that described methods of handling protocol deviations in publication.
Unacknowledged discrepancies between protocols and publications were found for sample size calculations (18/34 trials), methods of handling protocol deviations (19/43) and missing data (39/49), primary outcome analyses (25/42), subgroup analyses (25/25), and adjusted analyses (23/28).
All the animals handling protocol was approved by the Animal Research Ethics Committee of the University (Ref. No: 04/025/ERG; CUHK 4360/04 M).
Indeed, further refinement of animal handling protocols, implementation of new anaesthetic regimes and development in monitoring equipment are needed to maximise the potential of current preclinical imaging systems, minimising any confounding effects on the imaging outcomes and the studied biological processes.
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