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BKC and SR carried out the handling of cell lines, maintaining cell culture, MTT assay, CV staining, and data interpretation along with discussion and conclusion.
The latest products from Eppendorf deliver exceptional levels of product purity and security, as well as improved, ergonomic and safe handling of cell cultures and advanced protection against contamination.
Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition).
The constancy of cell types and numbers and the epithelial integrity in adult tissues relies on the rapid and accurate handling of cell loss by their resident multipotent stem cells.
Differences between 1 g ground and 1 g in-flight controls can be attributed to the flight itself (e.g., vibrations, handling of cell containers) and not to an altered gravity.
This would allow a more flexible handling of cell cultures adapted to the demand, e.g. a rapid upscaling of cultures after storage, i.e. keeping cells in "stand-by" storage.
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This discrepancy in our opinion could have been attributed to the stress that might have occurred during handling of cells while harvesting the cells for the assay.
Many were likely present in the original skin cells, and some could have arisen during the handling of cells before they were reprogrammed.
Here we exploit the deformability of the cytoskeleton as a link between molecular structure and biological function, to distinguish between cells in different states by using a laser‐based optical stretcher (OS) coupled with microfluidic handling of cells.
We further discuss the application of acoustic-based microfluidic systems for the mixing and transport of liquids, manipulation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules, followed by explanation on the present challenges of acoustic-based microfluidics for the handling of cells and molecules, and highlighting the future directions.
This procedure describes the handling of cells on thin-bottom 96-well dishes.
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