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The aim of the present study was, therefore, to determine the effect of chronic furosemide application on renal Mg2+ handling in mice.
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To check whether tranilast administration altered glucose handling in control and dystrophic mice we also performed a glucose tolerance test.
In turn this was related to altered calcium handling in the heart tissue of PGC1β mice.
Recent studies have linked osteocalcin to improved glycaemic handling in tissues[ 31], and osteocalcin knockout mice exhibit marked insulin resistance and an adverse metabolic phenotype[ 32].
Control mice were handled in parallel with experimental mice but not exposed to females with the exception of the initial cohabitation period during day -7 to day 0 of the experiment.
For 3 days before testing, mice were weighed and handled in order to habituate the mice to contact with the experimenter.
The ability to elicit seizures through daily handling in SynDKO mice undergoing electrophysiological recording is a significant improvement in comparison to the traditional cable-based set-up.
These data show an alteration in glucose handling in SOD1G86R mice at the asymptomatic stage of disease.
Six- to eight-week-old nu/nu (Ncr) athymic mice, handled in accordance with approved Institutional Animal Care and Use Committee protocols, were used for xenograft studies.
The Research Ethics Committee of Jilin University reviewed and approved all the procedures for handling the mice used in this study.
All procedures for handling the mice used in this study were reviewed and approved by the Institute of Laboratory Animal Resources of Seoul National University and performed in an ethical and humane manner under veterinary supervision.
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