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The stress response seen after 30 min may be a result of disturbed social contact with cage mates but can also be a handling effect or induced by exposure to a novel environment.
The automaker said the effect would vary depending on the number and location of the bolts, but initially "noise and a minor handling effect may be noticed".
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Pythium were infrequently detected likely because of soil handling effects.
In a dyadic encounter with another male mouse, subjects of both lines showed handling effects.
Collecting and/or handling effects are likely to be minimal for all elements in our study based on similar treatment of fish after capture, chemical similarity, and the sterilized conditions.
Sensitivity of birds to the translocation procedure has to be rather low, as handling effects might otherwise overrule experimental effects on breeding behaviour.
Each injected male was placed in an 80 L tank adjacent to another tank containing five conspecific females of various sizes, adding 8 mL of Stress Coat (Aquarium Pharmaceuticals) to mitigate handling effects.
We also noted some physical dissection and handling effects on fragment size.
Similar handling effects were seen by Maynard et al. (2004) when gentle air currents in the laboratory produced airborne single-walled carbon nanotube particles.
Additional studies directed at normalizing the cellular physiologic homeostasis of individual donor samples before experimentation would allow distinction between genetically endowed differences and treatment/ handling effects.
Further automatization that obviates human contact might further decrease variability by removing handling effects, thus allowing for virtually complete standardization between laboratories.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com