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This may be attributed to use of different donors, antigens tested or protocol properties for thawing, handling and testing the cells in ELISPOT.
The collection, handling, and testing of samples in this study were identical to one of these previous studies [ 2].
To simulate the time and manner by which proteins could be exposed to repetitive cycles of freezing during typical handling and testing processes, the purified protein was subjected to 3 freeze/thaw cycles while being exposed to ambient temperature after each thaw cycle.
For easy handling and testing with the PTFE sheet, we have used a large cylindrical plastic tube wrapped with a PTFE sheet (12 cm long, 5 cm wide, and 70 μm thick) as illustrated in Figure 2c.
After recovery from surgery (1 week), the animals were acclimated to all of the handling and testing procedures described previously.
Additional laboratory space was identified, consisting of three separate areas for handling and testing bioterrorism samples (two polymerase chain reaction [PCR] units and an enzyme immunoassay [EIA] rapid screening unit).
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The presence of agarose yields robust systems that can be handled and tested as prepared, in the fresh state.
With a special plug-in, some products can be viewed in interactive 3-D, which lets you "handle" and test them as you would in the store.
The DEA must approve researchers' licenses to handle and test marijuana, which is considered illegal under federal law, and must dole out the drug for research from a government stash.
Animals were gently handled and tested in a quiet environment to minimize stress levels.
On day 2, rats were briefly handled and tested for 'time-out' (see below).
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