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In fact, immunohistochemistry is semiquantitative and highly dependent on a range of variables, such as choice of antibody, antibody concentration, fixation techniques, variability in the interpretation and stratification criteria, and inconsistency in specimen handling and technical procedures.
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2334/14/39/prepub We sincerely thank Patricia Vierhaus, Department of Medical Informatics, Statistics and Epidemiology, University of Bochum, Germany, for data handling and technical assistance.
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