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The imaged larvae were allowed to develop to adulthood to ensure that handling and imaging did not interfere with normal development.
All animal handling and imaging procedures were approved by the University of Notre Dame Institutional Animal Care and Use Committee.
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Second, the two-dimensional nature of the sample and the resiliency of fixed skin simplifies tissue handling, imaging, and cataloguing of structures by their location in the X Y plane.
In some cases, the combination of specimen handling, imaging and feature extraction can be very rapid.
The issue of animal handling when imaging multiple mice is further discussed in section entitled "Issues related to animal handling".
Despite repeated handling, transport and imaging sessions, images of the phantoms taken at UCL at the start and end of the project (Nov 2015 Mar 2017) were indistinguishable by eye from each other, suggesting that the properties of the phantoms had not changed substantially during the 18 months or so of the study.
Particularly, handling and restraint of awake rats have been shown to influence the DA system and affect the binding potential of PET imaging tracers [ 7, 40].
Because the skeleton is lightly crushed and bones cannot be handled individually, imaging studies are of particular importance.
To ensure that such research continues, we must develop informatics infrastructures that meet research needs, one of which is an easily implementable comprehensive translational research database capable of handling imaging.
We designed a relational database (DB) to store information pertaining to all cells in a given video ('Materials and methods', Long-terms time-lapse imaging and Data handling and image processing).
The final session covered the handling, analysis and imaging of tissue in the laboratory.
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