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Other studies often do not explicitly mention how this is handled as for instance Spijker et al 2008 [ 16].
For protein O-glycation and phosphorylation studies on HUVEC, cells were handled as for viability assays, except that after 24 hours of incubation supernatants and floating cells were removed and adherent cells lysed as described above.
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In certain situations a function v which depends on the unknown function u can be handled as well (see, for example, [8]).
Revlon Foundation "The size of this tool makes it perfect for handling — as well as for travel.
It is the job of machines to handle as much work for humans as possible, so as to free them to pursue that which each and every individual human being most wishes to pursue.
For Western blot analysis, cells were handled as described for the cell spreading assay; cells were lysed directly by the addition of SDS PAGE loading buffer to the dish.
Except for the TEX treatment, the libraries were handled as described for the treated libraries.
For the analysis of outcomes, missing data were handled as follows: for NRT use, blank cells indicating possible missing data were completed or not according to the context, which was usually daily application of a nicotine patch.
On the following day, myoblast cells were transfected with vinculin expression constructs using Nanofectin transfection reagent (PAA) according to the manufacturer's instructions and subsequently handled as detailed for MEFs (see above).
The samples were handled as described for the experiment in orbit.
Formalin-fixed spleen tissues were handled as described for kidneys, with Prussian blue staining used to measure iron accumulation.
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