The L.A. policy raises questions about how Metro will handle stations that already bear the name of institutions that have not paid for naming rights, including the Duarte/City of Hope station on the Gold Line and two Expo Line stations adjacent to USC.
All experimental tools were developed using the robotic liquid handling station Tecan Freedom Evo 200.
A method to generate phase diagrams in high throughput on an automated liquid handling station in microbatch scale was developed.
Several PEG/phosphate systems varying in PEG molecular weight, pH and NaCl concentration were investigated in an automated small scale screening performed on a liquid handling station.
Densities were measured by a standalone micro fluidic liquid density sensor, which was integrated into a liquid handling station by means of a developed generic Tip2World interface.
Results are presented from the optimal operation of a fully automated robotic liquid handling station where parallel experiments are performed for calibrating a kinetic fermentation model.
DNA extractions were performed with the NucleoSpin96 (Machery-Nagel) kit according to the specification of the manufacturer under automation with a Biomek NX liquid-handling station (Beckman-Coulter) equipped with a filtration manifold as previously described [40], [41].
Several methods were used throughout the duration of the project: Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich), or in-house developed protocols Chelex-based 'DryRelease', silica-based 'Silitom' [43] and automated DNA extraction protocol on the Biomek FX© liquid handling station using 1.0 µm PALL glass fiber media filter plates [44].
The entire procedure was automatically performed by the liquid handling station.
The pre-treatment, hydrolysis, and sugar determination of samples was performed using an automated liquid handling station (Tecan, Maennedorf, Switzerland).
Experiments were run in triplicate, using 384-well reaction plates in an automatic liquid handling station (epMotion 5075LH; Eppendorf).
