(A – C ) Serial EM images of rod outer segments were segmented by hand and reconstructed in TrakEM2.
With assistance from the RECA-1 channel, the microvessel was segmented by hand prior to summation of the Aqp4 signal and normalization to the microvessel area.
The α-Al dendritic structure was segmented by hand into sub-volumes of 350 × 220 × 200 voxels (0.4 mm), which had been previously filtered by a 2-D anisotropic diffusion filter [24] to improve the quality of the reconstructed slices.
To calculate the nuclear-to-cytoplasmic ratio at each ROI in the hematoxylin-eosin image, the digital images were segmented by hand and converted to a binary image, with white nuclei and black cytoplasm.
Overlaid is a hand-rendered 3D representation of MTs (green) (ii ) and mesh segmented by automated detection (yellow) (iii ).
Operations are segmented by solutions provided.
AOEs were segmented by an anatomist (N.M).
In total 798 hand segments in SSc were scored, where 251 hand segments (31.5%) revealed an enhancement of ICG.
At baseline, 31.5% hand segments showed ICG enhancement.
All 38 segments from both hands were analyzed and ICG fluorescence enhancement was determined per hand, and the total sum score from both hand segments was archived.
Each session was recorded continuously and then hand-segmented by the authors into segments lasting between 2 60 s.
