To check whether viral amplification is not hampered by using the non-rRNA-hexamers for cDNA synthesis we performed real-time PCRs on cDNA of HCoV-NL63, echovirus 18, and human coxsackievirus A16 virus culture supernatant.
Analysis of surgical procedures is hampered by the use of small subject groups, the infrequent use of a control group, the unmatched selection of patients of varying ages, and the varying severity of the disease process.
Although promising results have been obtained in protection assays against amoebiasis using various experimental models such as mice, hamsters, and gerbils, the use of these strategies to protect humans in the future is hampered by the use of adjuvants that are potentially toxic and proinflammatory to mammals, such as bacterial toxins or oil-based adjuvants.
Direct comparisons of results among studies were hampered by the use of different statistics (medians, means, or geometric means) and sampling strategies; the use of standardized methods could strengthen the validity of results and facilitate comparisons among different populations and locations.
The interaction between patients and physicians may be hampered by the use of a PDA during the patient encounter, but explaining the reason for using a PDA to the patient was found to have a positive effect on patient-physician interactions and communications [ 122].
Statistical computation in such settings is hampered by the use of look-up tables or expensive 'black-box' function evaluations.
According to the report, communication among assisting organizations was also a problem, hampered by the use of "10-codes" instead of plain language.
Unfortunately, the success of ITN campaigns is hampered by improper use and natural decay in ITN-efficacy over time.
Outbreaks of cholera are frequent, and potential earnings from tourism are hampered by the use of the city's main beaches as a public toilet.
Comparability is currently hampered by the use of abundance statistics that do not estimate a meaningful parameter of the microbial community and biases introduced by experimental protocols and data-cleaning approaches.
