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This layer may hinder fluorophore penetration into the epithelial cells of the underlying strata and therefore hamper imaging with CellVizio®.
However, several factors may hamper imaging of the pancreas.
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Many of the problems relate to their small size, which is less than the wavelength of visible light and hampers imaging by light microscopy.[ 8] For example, while the standard method of production (extrusion through a microporous filter) allows control over size, a range of diameters are always present in a given sample.
Optic disc imaging is a useful way to document the glaucoma status in this type of eye, provided that media opacification does not hamper image acquisition.
Modern PET cameras have high sensitivity, but at very low cellular concentration levels, the limiting physical factors inherent to the PET technique [15 17] as well as the specific biological and pharmacological properties of the Ga68-PSMA tracer hamper tumor imaging performance.
Low radiolabelling efficiency (<95%) of radiopharmaceutical is problematic, with any unlabelled material competing for target, hampering quantitative measurements and imaging sensitivity, and requiring an additional purification step prior to imaging.
However, the PSMA expression in other tissues such as the human brain [11] hampers this approach to PCa imaging.
The direct assessment of changes in aneurysm haemodynamics after stent deployment is hampered by limitations in existing imaging techniques and currently requires resorting to numerical simulations.
Inflammation in healing myocardium is one such therapeutic target, though clinical exploration has been hampered by the absence of imaging biomarkers.
The embryo has a large amount of fluorescent yolk, which hampers fluorescence imaging because of their spectral overlaps.
However, the limited penetration of the external excitation light and the strong tissue autofluorescence hampered the imaging of the deeper tissues, such as liver, and caused high background emissions.
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